The PCR product should be 25 ml (if purified) - 50 ml (if not purified).The picture should be taken from the DNA sample by running it in 2 ml of agarose gel, and the picture should be submitted with the sample. The DNA sample should be at a concentration of at least 100 ng/ml and a volume of 20 ml.For sequence analysis, a DNA sample or PCR product of the region to be sequenced must be provided.Persons who will order DNA sequence analysis and/or DNA fragment analysis (Microsatellite or AFLP) should carefully read the following explanations. IMPORTANT EXPLANATIONS ON ANALYSIS REQUESTS FOR ABI 310 DNA ANALYZER For details on the delivery of samples, please read the explanations below.
The quality of the data depends on the amount and purification of the DNA used in sample production. During the preparation of each capillary-loaded sample, the fluorescently labeled size standard is added to ensure that the results of determining the length of the PCR products are consistent. In genotype determination, the multi-color detection system provides the opportunity to simultaneously examine more than one PCR product of various sizes marked with different colors. Due to this feature, the sequence analysis reaction is carried out in a single reaction tube. Up to 450-500 bases of DNA can be read with a 45 cm capillary, and the processing time is shortened by approximately 1 hour per sample compared to a 61 cm capillary.ĭNA sequencing is done using the BigDye Dideoxy Terminators system.ĥ' fluorescent-labeled primers should be used for DNA fragment analysis (microsatellite and AFLP).ĪBI 310 Genetic Analyzer can detect five fluorescent colors simultaneously in the same capillary. With the 61 cm silica capillary, up to 650 bases of DNA can be read, and ten samples can be examined per day. Sequence analysis and DNA fragment analysis have become quite easy with the fluorescent color detection system. This device automates the gel pouring and sample loading processes. ABI 310 is a DNA analysis device based on capillary electrophoresis.